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Extrationless vs. Automated: The Science Behind PCR Precision

A comprehensive technical analysis of inhibitor impact, workflow complexity, and performance metrics comparing extractionless and automated extraction

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The Inhibitor Problem

Explore how inhibitors like heme, mucins, urea, and bile salts interfere with amplification-altering Ct values, masking low-copy targets, and compromising quantitative accuracy across sample types.

Hidden Workflow Complexity

See the step-by-step breakdown of both methods, including aliquoting, centrifugation, heat treatment, and bead-beating requirements that reveal the true complexity of extractionless workflows.

Head-to-Head Technical Data

Review detailed comparison metrics including processing time, manual steps, nucleic acid recovery rates, contamination risk, and the science behind magnetic bead technology's >98% recovery consistency.

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Download the Complete Technical Analysis

Access the full whitepaper with in-depth inhibitor mechanisms, complete workflow protocols, magnetic bead specs, and long-term cost-benefit calculations.

Get the Complete Whitepaper